Pretreatment of lignocellulosic biomass by breaking down its complex structure is an essential step to facilitate enzymatic hydrolysis of lignocellulose and subsequent bioethanol fermentation. Herein, an eco-friendly pretreatment strategy was developed by applying deep eutectic solvent (DES) cocktail. Attributing to the synergistic effect of two DESs (e.g., betaine-glycerol and choline chloride-oxalic acid (or choline chloride-acetamide)), an impressive 89.51% digestibility for glucan and 75.43% for xylan were achieved. Characterization of pretreated corn stover showed that DES cocktail changed substrate surface structure and enlarged structure pores, which could improve the accessibility of cellulase. The pretreated corn stover was also employed in ethanol fermentation and achieved the yield of 73.35%. Recycling experiments demonstrated the potential reusability of DES cocktail in a sustainable manner towards various biomass sources. These findings highlight the effectiveness and versatility of the developed DES cocktail pretreatment method, paving the way for sustainable bioethanol production from lignocellulosic feedstocks.

中文翻译：

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低共熔溶剂混合物提高木质纤维素的预处理效率


通过分解木质纤维素生物质的复杂结构对其进行预处理是促进木质纤维素酶水解和随后的生物乙醇发酵的重要步骤。在此，通过应用低共熔溶剂（DES）混合物开发了一种环保的预处理策略。由于两种 DES（例如甜菜碱-甘油和氯化胆碱-草酸（或氯化胆碱-乙酰胺））的协同作用，葡聚糖的消化率达到了令人印象深刻的 89.51%，木聚糖的消化率达到了 75.43%。预处理玉米秸秆的表征表明，DES 混合物改变了基质表面结构并扩大了结构孔，从而提高了纤维素酶的可及性。预处理后的玉米秸秆也用于乙醇发酵，收率达到73.35%。回收实验证明了 DES 混合物以可持续的方式对各种生物质来源具有潜在的可重复使用性。这些发现凸显了所开发的 DES 鸡尾酒预处理方法的有效性和多功能性，为利用木质纤维素原料可持续生产生物乙醇铺平了道路。