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低强度脉冲超声通过激发压电BaTiO 3 / TC4改善MC3T3-E1细胞的活性
Bioactive Materials
(
IF
18.9
)
Pub Date : 2021-04-21
, DOI:
10.1016/j.bioactmat.2021.04.016
Kunzhan Cai
,
Yilai Jiao
,
Quan Quan
,
Yulin Hao
,
Jie Liu
,
Lin Wu
多年来,开发用于骨植入物以增强骨愈合和骨生长的生物活性材料一直是临床研究的重点。钛酸钡(BT)是一种电活性材料,可以响应施加的机械力而产生电信号。在这项研究中,在TC4钛合金的表面上合成了BT压电陶瓷涂层,形成BT / TC4材料,然后将低强度脉冲超声(LIPUS)用作机械刺激。研究了对MC3T3-E1细胞生物学反应的综合影响。扫描电子显微镜,能量色散X射线光谱和X射线衍射的结果表明,在TC4基底上形成了均匀的纳米球状的BT涂层。33 of 0.42 pC / N。电化学测量表明,LIPUS刺激的BT / TC4材料可产生约10μA/ cm 2的微电流。在体外,当使用LIPUS刺激BT / TC4时,在MC3T3-E1细胞中发现了最大的成骨作用(细胞粘附,增殖和成骨分化)。此外,这些细胞中细胞内钙离子浓度增加,可能是由于促进了L型钙离子通道的开放和Ca V 1.2蛋白的表达增加。因此,具有LIPUS负载的压电BT / TC4材料可协同促进成骨作用,使其成为早期形成可靠的骨-植入物接触的潜在方法。
"点击查看英文标题和摘要"
Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound
Developing bioactive materials for bone implants to enhance bone healing and bone growth has for years been the focus of clinical research. Barium titanate (BT) is an electroactive material that can generate electrical signals in response to applied mechanical forces. In this study, a BT piezoelectric ceramic coating was synthesized on the surface of a TC4 titanium alloy, forming a BT/TC4 material, and low-intensity pulsed ultrasound (LIPUS) was then applied as a mechanical stimulus. The combined effects on the biological responses of MC3T3-E1 cells were investigated. Results of scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction showed that an uniform nanospheres -shaped BT coating was formed on TC4 substrate. Piezoelectric behaviors were observed using piezoelectric force microscopy with the piezoelectric coefficient d33 of 0.42 pC/N. Electrochemical measures indicated that LIPUS-stimulated BT/TC4 materials could produce a microcurrent of approximately 10 μA/cm2. In vitro, the greatest osteogenesis (cell adhesion, proliferation, and osteogenic differentiation) was found in MC3T3-E1 cells when BT/TC4 was stimulated using LIPUS. Furthermore, the intracellular calcium ion concentration increased in these cells, possibly because opening of the L-type calcium ion channels was promoted and expression of the CaV1.2 protein was increased. Therefore, the piezoelectric BT/TC4 material with LIPUS loading synergistically promoted osteogenesis, rending it a potential treatment for early stage formation of reliable bone-implant contact.
更新日期:2021-04-21