SPARCL1 Polyclonal antibody
SPARCL1 Polyclonal Antibody for IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
IHC, IP, WB, ELISA and More (1)
Conjugate
Unconjugated
Publications
8
Cat No : 13517-1-AP
Print datasheet
Synonyms
验证数据展示
经过测试的应用
Positive WB detected in | human heart tissue |
Positive IP detected in | Raji cells |
Positive IHC detected in | human tonsillitis tissue, human gliomas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 4 publications below |
IHC | See 2 publications below |
IF | See 1 publications below |
产品信息
13517-1-AP targets SPARCL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | IHC, IP, WB, ELISA |
Cited Applications | WB, IF, IHC |
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Immunogen | SPARCL1 fusion protein Ag4448 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | SPARC-like 1 (hevin) |
Synonyms | Hevin, MAST 9, PIG33, SC1, SPARC like 1 (hevin), SPARC like protein 1, SPARCL1 |
Calculated Molecular Weight | 664 aa, 75 kDa |
Observed Molecular Weight | 130-140 kDa |
GenBank Accession Number | BC033721 |
Gene Symbol | SPARCL1 |
Gene ID (NCBI) | 8404 |
RRID | AB_10598475 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q14515 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for SPARCL1 antibody 13517-1-AP | Download protocol |
IHC protocol for SPARCL1 antibody 13517-1-AP | Download protocol |
IP protocol for SPARCL1 antibody 13517-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
- All (8)
- WB (4)
- IHC (2)
- IF (1)
Species | Application | Title |
---|---|---|
CNS Neurosci Ther Endothelial genetic deletion of CD147 induces changes in the dual function of the blood-brain barrier and is implicated in Alzheimer's disease. View Article | ||
J Neurochem Defective Synthesis and Release of Astrocytic Thrombospondin-1 Mediates the Neuronal TDP-43 Proteinopathy Resulting in Defects in Neuronal Integrity Associated with Chronic Traumatic Encephalopathy: In Vitro Studies. View Article | ||
Cancer Biomark Integrated analysis reveals down-regulation of SPARCL1 is correlated with cervical cancer development and progression View Article | ||
Oral Dis FERMT2 upregulation in CAFs enhances EMT of OSCC and M2 macrophage polarization View Article | ||
PLoS One Secreted protein acidic and rich in cysteine (SPARC) knockout mice have greater outflow facility. View Article | ||
J Chem Neuroanat Olfactory ensheathing cells facilitate neurite sprouting and outgrowth by secreting high levels of hevin. View Article |