- Featured Product
- KD/KO Validated
MCM6 Polyclonal antibody
MCM6 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
Publications
21
Cat No : 13347-2-AP
Print datasheet
Synonyms
验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HeLa cells, mouse ovary tissue, mouse thymus tissue, mouse spleen tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human cervical cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy MCM6 Ready-To-Use IHC Kit. MCM6 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 3 publications below |
| WB | See 16 publications below |
| IHC | See 8 publications below |
| IF | See 2 publications below |
产品信息
13347-2-AP targets MCM6 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | MCM6 fusion protein Ag4180 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | minichromosome maintenance complex component 6 |
| Synonyms | MCG40308, MCM6, Mis5, P105MCM |
| Calculated Molecular Weight | 821 aa, 93 kDa |
| Observed Molecular Weight | 105 kDa |
| GenBank Accession Number | BC032374 |
| Gene Symbol | MCM6 |
| Gene ID (NCBI) | 4175 |
| RRID | AB_2142537 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14566 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes[PMID: 18096807]. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells[PMID: 9305914]. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities [PMID:21336027]. The calculated molecular weight of MCM6 is 92 kDa, but the modified MCM6 is about 105 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for MCM6 antibody 13347-2-AP | Download protocol |
| IHC protocol for MCM6 antibody 13347-2-AP | Download protocol |
| IF protocol for MCM6 antibody 13347-2-AP | Download protocol |
| IP protocol for MCM6 antibody 13347-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
- All (21)
- KD/KO (3)
- WB (16)
- IHC (8)
- IF (2)
| Species | Application | Title |
|---|---|---|
J Hematol Oncol Proteogenomic insights into the biology and treatment of pancreatic ductal adenocarcinoma View Article | ||
Biomaterials Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis. View Article | ||
Elife MCMBP promotes the assembly of the MCM2-7 hetero-hexamer to ensure robust DNA replication in human cells. View Article | ||
Front Oncol METTL3 Promotes the Progression of Gastric Cancer via Targeting the MYC Pathway. View Article | ||
Oncotarget Lamin B2 binding to minichromosome maintenance complex component 7 promotes non-small cell lung carcinogenesis. View Article | ||
Ecotoxicol Environ Saf The influence of phenolic environmental estrogen on the transcriptome of uterine leiomyoma cells: A whole transcriptome profiling-based analysis. View Article |
