PFKFB3 Polyclonal antibody

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IF/ICC, IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Publications

107

Cat No : 13763-1-AP

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Synonyms

6PF 2 K/Fru 2,6 P2ase 3, IPFK2, PFK/FBPase 3, PFK2, PFKFB3, PFKFB3-Specific

概述

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MSDS

WB analysis using 13763-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 13763-1-AP
WB result of PFKFB3-Specific antibody (13763-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PFKFB3-Specific transfected HEK-293 cells.
IF Staining of HepG2 using 13763-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PFKFB3-Specific antibody (13763-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IP experiment of HEK-293 using 13763-1-AP
IP result of anti-PFKFB3 (IP:13763-1-AP, 3ug; Detection:13763-1-AP 1:1000) with HEK-293 cells lysate 4000ug.
IHC staining of human liver cancer using 13763-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 13763-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13763-1-AP (PFKFB3-Specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Size

Positive WB detected in	HEK-293 cells, mouse thymus tissue, mouse heart tissue, A431 cells, HeLa cells, Jurkat cells, mouse spleen tissue
Positive IP detected in	HEK-293 cells, mouse spleen tissue
Positive IHC detected in	human liver cancer tissue, human kidney tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HepG2 cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

13763-1-AP targets PFKFB3 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	IF/ICC, IHC, IP, WB, ELISA
Cited Applications	WB, IP, IF, FC, IHC, CoIP
Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat
Immunogen	PFKFB3 fusion protein Ag4744 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3
Synonyms	6PF 2 K/Fru 2,6 P2ase 3, IPFK2, PFK/FBPase 3, PFK2, PFKFB3, PFKFB3-Specific
Calculated Molecular Weight	520 aa, 60 kDa
Observed Molecular Weight	58 kDa
GenBank Accession Number	BC040482
Gene Symbol	PFKFB3
Gene ID (NCBI)	5209
RRID	AB_2162854
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q16875
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Product Specific Protocols
WB protocol for PFKFB3 antibody 13763-1-AP	Download protocol
IHC protocol for PFKFB3 antibody 13763-1-AP	Download protocol
IF protocol for PFKFB3 antibody 13763-1-AP	Download protocol
IP protocol for PFKFB3 antibody 13763-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
mouse	WB	Nature FGF-dependent metabolic control of vascular development. Authors - Pengchun Yu View Article
human	WB	Cell Role of PFKFB3-Driven Glycolysis in Vessel Sprouting. Authors - De Bock Katrien K View Article
human	WB	Cell Metab CircACC1 Regulates Assembly and Activation of AMPK Complex under Metabolic Stress. Authors - Qidong Li View Article
human	WB,IP	Cell Metab NEAT1 is essential for metabolic changes that promote breast cancer growth and metastasis. Authors - Mi Kyung Park View Article
mouse	WB	Circulation Exercise-Induced Changes in Glucose Metabolism Promote Physiologic Cardiac Growth. Authors - Andrew A Gibb View Article
mouse	WB	ACS Nano Dual Inhibition of Endoplasmic Reticulum Stress and Oxidation Stress Manipulates the Polarization of Macrophages under Hypoxia to Sensitize Immunotherapy. Authors - Mengshi Jiang View Article