Featured Product
KD/KO Validated

PEX14 Polyclonal antibody

PEX14 Polyclonal Antibody for FC, IF/ICC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, monkey and More (2)

Applications

FC, IF/ICC, IHC, IP, WB, ELISA

Conjugate

Unconjugated

Publications

Cat No : 10594-1-AP

Print datasheet

Synonyms

Cell and organelle markers, NAPP2, Peroxin 14, Peroxisome Marker, PEX14, Pex14p, PTS1 receptor docking protein

概述

经过测试的应用
产品信息
发表文章 (55)
实验方案
相关产品 (5)

Print datasheet

MSDS

验证数据展示

WB analysis of HEK-293 using 10594-1-AP
WB result of PEX14 antibody (10594-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PEX14 transfected HEK-293 cells.
WB analysis using 10594-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10594-1-AP (PEX14 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
IHC staining of mouse liver using 10594-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10594-1-AP (PEX14 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 10594-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10594-1-AP (PEX14 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10594-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PEX14 antibody (10594-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), (CL594-Phalloidin, red).
IP experiment of mouse liver using 10594-1-AP
IP result of anti-PEX14 (IP:10594-1-AP, 3ug; Detection:10594-1-AP 1:500) with mouse liver tissue lysate 4000ug.
FC experiment of HeLa using 10594-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PEX14 (10594-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

-/ -

Cat No. 10594-1-AP

Size

点击咨询在线客服

经过测试的应用

Positive WB detected in	A431 cells, HEK-293 cells, HEK-293T cells, HeLa cells, SKOV-3 cells
Positive IP detected in	mouse liver tissue
Positive IHC detected in	mouse liver tissue, human liver cancer tissue, human cervical cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	HeLa cells, COS7 cells, HepG2 cells, NIH/3T3 cells, C2C12 cells
Positive FC detected in	HeLa cells

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)	IF : 1:500-1:2000
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 1 publications below
WB	See 17 publications below
IHC	See 5 publications below
IF	See 39 publications below

产品信息

10594-1-AP targets PEX14 in WB, IP, IF, FC, IHC, ELISA applications and shows reactivity with human, mouse, rat, monkey samples.

Tested Applications	FC, IF/ICC, IHC, IP, WB, ELISA
Cited Applications	WB, IF, IHC
Tested Reactivity	human, mouse, rat, monkey
Cited Reactivity	human, mouse, rat, chicken, yeast, monkey
Immunogen	PEX14 fusion protein Ag0932 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	peroxisomal biogenesis factor 14
Synonyms	Cell and organelle markers, NAPP2, Peroxin 14, Peroxisome Marker, PEX14, Pex14p, PTS1 receptor docking protein
Calculated Molecular Weight	41 kDa
Observed Molecular Weight	57 kDa
GenBank Accession Number	BC006327
Gene Symbol	PEX14
Gene ID (NCBI)	5195
RRID	AB_2252194
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75381
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

PEX14 (peroxisomal biogenesis factor 14) is a peroxisomal membrane protein that is essential for protein docking onto the peroxisomes. It is a central component of the peroxisimal matrix protein import machinery and interacts with PEX5 and PEX19. PEX14 is ubiquitously expressed and defects in PEX14 are the cause of peroxisome biogenesis disorder complementation group K (PBD-CGK). This antibody can be used to detect endogenous PEX14 with an apparent molecular weight of 57 kDa (PMID: 16449325; 9653144) and recognize peroxisomal structures in human, monkey and mouse cells.

实验方案

Product Specific Protocols
WB protocol for PEX14 antibody 10594-1-AP	Download protocol
IHC protocol for PEX14 antibody 10594-1-AP	Download protocol
IF protocol for PEX14 antibody 10594-1-AP	Download protocol
IP protocol for PEX14 antibody 10594-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

All (55)
KD/KO (1)
WB (17)
IHC (5)
IF (39)

Species	Application	Title
human		Science Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division. Authors - Shun Nagashima View Article
mouse	IF	Cell A Network of Macrophages Supports Mitochondrial Homeostasis in the Heart. Authors - José A Nicolás-Ávila View Article
human	IF	Mol Cell The HRI branch of the integrated stress response selectively triggers mitophagy Authors - Yogaditya Chakrabarty View Article
mouse	IF	Mol Cell Drp1 Tubulates the ER in a GTPase-Independent Manner. Authors - Yoshihiro Adachi View Article
mouse	WB, IHC	Cell Metab Hif-2α promotes degradation of mammalian peroxisomes by selective autophagy. Authors - Katharina M Walter View Article
human	WB	Nat Chem Biol Peroxisomal-derived ether phospholipids link nucleotides to respirasome assembly. Authors - Christopher F Bennett View Article