pGL4.18[luc2P/Neo]

LOCUS       pGL4.18[luc2P Neo]                5727 bp ds-DNA     circular SYN 29-JUN-2013
DEFINITION  Promoterless vector encoding destabilized luciferase for measuring 
            the activity of promoter and enhancer sequences.
ACCESSION   DQ188838
VERSION     .
KEYWORDS    pGL4.18[luc2P Neo]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Promega
  TITLE     Direct Submission
  JOURNAL   Exported Monday, February 17, 2020 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     misc_feature    1..70
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..1749
                     /codon_start=1
                     /product="firefly luciferase"
                     /note="luciferase"
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     CDS             1753..1872
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /note="hPEST"
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    1924..2045
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        2239..2596
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2627..3421
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /note="NeoR"
                     /note="confers resistance to neomycin, kanamycin, and  G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3446..3494
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(3821..4409)
                     /direction=LEFT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4609..5469)
                     /codon_start=1
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     polyA_signal    5574..5622
                     /note="synthetic polyadenylation signal"
     misc_feature    5636..5727
                     /note="pause site"
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
ORIGIN
        1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
       61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
      121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
      181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
      241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
      301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
      361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
      421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
      481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
      541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
      601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
      661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
      721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
      781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
      841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
      901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
      961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
     1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
     1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
     1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
     1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
     1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
     1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
     1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
     1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
     1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
     1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
     1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
     1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
     1741 atcgccgtga attctcacgg cttccctccc gaggtggagg agcaggccgc cggcaccctg
     1801 cccatgagct gcgcccagga gagcggcatg gatagacacc ctgctgcttg cgccagcgcc
     1861 aggatcaacg tctaaggccg cgactctaga gtcggggcgg ccggccgctt cgagcagaca
     1921 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     1981 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     2041 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     2101 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtttgcgt
     2161 attgggcgct cttccgctga tctgcgcagc accatggcct gaaataacct ctgaaagagg
     2221 aacttggtta gctaccttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg
     2281 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     2341 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     2401 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     2461 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     2521 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     2581 cctaggcttt tgcaaaaagc tcgattcttc tgacactagc gccaccatga tcgaacaaga
     2641 cggcctccat gctggcagtc ccgcagcttg ggtcgaacgc ttgttcgggt acgactgggc
     2701 ccagcagacc atcggatgta gcgatgcggc cgtgttccgt ctaagcgctc aaggccggcc
     2761 cgtgctgttc gtgaagaccg acctgagcgg cgccctgaac gagcttcaag acgaggctgc
     2821 ccgcctgagc tggctggcca ccaccggcgt accctgcgcc gctgtgttgg atgttgtgac
     2881 cgaagccggc cgggactggc tgctgctggg cgaggtccct ggccaggatc tgctgagcag
     2941 ccaccttgcc cccgctgaga aggtttctat catggccgat gcaatgcggc gcctgcacac
     3001 cctggacccc gctacctgcc ccttcgacca ccaggctaag catcggatcg agcgtgctcg
     3061 gacccgcatg gaggccggcc tggtggacca ggacgacctg gacgaggagc atcagggcct
     3121 ggcccccgct gaactgttcg cccgactgaa agcccgcatg ccggacggtg aggacctggt
     3181 tgtcacacac ggagatgcct gcctccctaa catcatggtc gagaatggcc gcttctccgg
     3241 cttcatcgac tgcggtcgcc taggagttgc cgaccgctac caggacatcg ccctggccac
     3301 ccgcgacatc gctgaggagc ttggcggcga gtgggccgac cgcttcttag tcttgtacgg
     3361 catcgcagct cccgacagcc agcgcatcgc cttctaccgc ttgctcgacg agttctttta
     3421 atgatctaga accggtcatg gccgcaataa aatatcttta ttttcattac atctgtgtgt
     3481 tggttttttg tgtgttcgaa ctagatgctg tcgaccgatg cccttgagag ccttcaaccc
     3541 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt
     3601 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc ttccgcttcc tcgctcactg
     3661 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     3721 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     3781 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     3841 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     3901 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     3961 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     4021 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     4081 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     4141 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     4201 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     4261 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     4321 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     4381 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     4441 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     4501 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     4561 agtaaacttg gtctgacagc ggccgcaaat gctaaaccac tgcagtggtt accagtgctt
     4621 gatcagtgag gcaccgatct cagcgatctg cctatttcgt tcgtccatag tggcctgact
     4681 ccccgtcgtg tagatcacta cgattcgtga gggcttacca tcaggcccca gcgcagcaat
     4741 gatgccgcga gagccgcgtt caccggcccc cgatttgtca gcaatgaacc agccagcagg
     4801 gagggccgag cgaagaagtg gtcctgctac tttgtccgcc tccatccagt ctatgagctg
     4861 ctgtcgtgat gctagagtaa gaagttcgcc agtgagtagt ttccgaagag ttgtggccat
     4921 tgctactggc atcgtggtat cacgctcgtc gttcggtatg gcttcgttca actctggttc
     4981 ccagcggtca agccgggtca catgatcacc catattatga agaaatgcag tcagctcctt
     5041 agggcctccg atcgttgtca gaagtaagtt ggccgcggtg ttgtcgctca tggtaatggc
     5101 agcactacac aattctctta ccgtcatgcc atccgtaaga tgcttttccg tgaccggcga
     5161 gtactcaacc aagtcgtttt gtgagtagtg tatacggcga ccaagctgct cttgcccggc
     5221 gtctatacgg gacaacaccg cgccacatag cagtactttg aaagtgctca tcatcgggaa
     5281 tcgttcttcg gggcggaaag actcaaggat cttgccgcta ttgagatcca gttcgatata
     5341 gcccactctt gcacccagtt gatcttcagc atcttttact ttcaccagcg tttcggggtg
     5401 tgcaaaaaca ggcaagcaaa atgccgcaaa gaagggaatg agtgcgacac gaaaatgttg
     5461 gatgctcata ctcgtccttt ttcaatatta ttgaagcatt tatcagggtt actagtacgt
     5521 ctctcaagga taagtaagta atattaaggt acgggaggta ttggacaggc cgcaataaaa
     5581 tatctttatt ttcattacat ctgtgtgttg gttttttgtg tgaatcgata gtactaacat
     5641 acgctctcca tcaaaacaaa acgaaacaaa acaaactagc aaaataggct gtccccagtg
     5701 caagtgcagg tgccagaaca tttctct
//