生物风
  • 首页
  • 技术服务
  • 试剂
  • 基因 · 蛋白 · 抗体
  • 载体
  • 细胞
  • 感受态 · 菌株
  • ATCC
注册 | 登录

pCS2TAL3-RR

价格:1000元

联系方式:I47-825O-882O

相关技术服务: 质粒构建     基因合成     质粒大提

买家导航

载体基本信息

质粒名称: pCS2TAL3-RR 
出品公司: 爱迪基因
目录编号: 37276
存储实验室: David Grunwald

载体骨架基本信息
载体名称: pCS2
载体抗性: 氨苄霉素
空载体大小: 4039 bp
载体修饰: The tal1c sequence from Dan Voytas lab's pTAL3 vector (1214-2210nt) consisting of a truncated N terminal and C terminal Tal protein regions and a LacZ screening module for use in Golden Gate cloning was PCR cloned into the KpnI and BamHI sites of the pCS2-Flag-GAAZFP-FokI-RR from Scot Wolfe's lab, making the new vector pCS2TAL3-RR. A Esp3I site in the FokI domain of the backbone vector was modified without changing the coding sequence.
载体类型: 哺乳细胞表达,Bacterial Expression
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
启动子 CMV
标签/融合蛋白:
HA (N terminal on backbone)
FokI RR (C terminal on backbone)
N-terminal Tal from pTAL3 (N terminal on backbone)
C terminal Tal From pTAL3 (C terminal on backbone)
克隆方法: 限制性内切酶
5' 测序引物 TTGGCGTCGGCAAACAGTGG
3' 测序引物 GGCAACGCGATGGGACGT

文献引用方法
材料和方法部分: pCS2TAL3-RR was a gift from David Grunwald (爱迪基因 plasmid # 37276)
参考文献部分: Simple Methods for Generating and Detecting Locus-Specific Mutations Induced with TALENs in the Zebrafish Genome. Dahlem TJ, Hoshijima K, Jurynec MJ, Gunther D, Starker CG, Locke AS, Weis AM, Voytas DF, Grunwald DJ. PLoS Genet. 2012 Aug;8(8):e1002861. Epub 2012 Aug 16. 10.1371/journal.pgen.1002861 PubMed 22916025

载体图谱质粒图谱和多克隆位点信息

pCS2TAL3-RR质粒图谱

载体简介

载体序列

LOCUS       pCS2TAL3-RR                5798 bp ds-DNA     circular SYN 10-JAN-2018
DEFINITION  TALEN backbone vector for use with the Golden Gate TALEN Kit in 
            place of pTAL1-4. Shorter N- and C-term tal protein segments 
            increases mutation induction; Fok I RR (improved version = 
            pCS2TAL3-RRR).
ACCESSION   .
VERSION     .
KEYWORDS    pCS2TAL3-RR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5798)
  AUTHORS   Dahlem TJ, Hoshijima K, Jurynec MJ, Gunther D, Starker CG, Locke AS,
            Weis AM, Voytas DF, Grunwald DJ
  TITLE     Simple Methods for Generating and Detecting Locus-Specific Mutations
            Induced with TALENs in the Zebrafish Genome.
  JOURNAL   PLoS Genet. 2012 Aug;8(8):e1002861. Epub 2012 Aug 16.
  PUBMED    22916025
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTNAME|pCS2TAL3-RR|
FEATURES             Location/Qualifiers
     source          1..5798
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        35..53
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     35..52
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     regulatory      85..94
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             97..117
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             118..207
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             complement(520..927)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGARLQGG
                     TRQCIARCMHCLHCGHAATFCNLHKLACVQL"
     primer_bind     777..799
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     791..808
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     792..808
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     818..837
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        818..836
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(884..902)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(923..939)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    947..963
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(971..1001)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1016..1037
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     CDS             1223..1810
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQAREMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     promoter        complement(1821..1838)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    1843..1977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1867..1886)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1921..1940
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(2094..2112)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2133..2149)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2157..2173
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2181..2211)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2226..2247
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2364..2381)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2535..3123)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2615..2634)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3294..4154)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3917..3936
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4155..4259)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4285..4740)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4422..4443)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4634..4653
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4814..5798
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
ORIGIN
        1 cgccattctg cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag
       61 ctacttgttc tttttgcagg atctgccacc atggctccaa agaagaagcg taaggtatac
      121 ccatacgatg ttcctgacta tgcgggctat ccctatgacg tcccggacta tgcaggatcg
      181 tatccatatg acgttccaga ttacgctgct catggtaccg tggatctacg cacgctcggc
      241 tacagtcagc agcagcaaga gaagatcaaa ccgaaggtgc gttcgacagt ggcgcagcac
      301 cacgaggcac tggtgggcca tgggtttaca cacgcgcaca tcgttgcgct cagccaacac
      361 ccggcagcgt tagggaccgt cgctgtcacg tatcagcaca taatcacggc gttgccagag
      421 gcgacacacg aagacatcgt tggcgtcggc aaacagtggt ccggcgcacg cgccctggag
      481 gccttgctca cggatgcggg ggagttgaga ggtccgccgt tacagttgga cacaggccaa
      541 cttgtgaaga ttgcaaaacg tggcggcgtg accgcaatgg aggcagtgca tgcatcgcgc
      601 aatgcactga cgggtgcccc cctggagacg ggcgccgcta cagggcgcgt cccattcgcc
      661 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      721 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      781 gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga
      841 attgggtacc gggccccccc tcgaggtcct ccagcttttg ttccctttag tgagggttaa
      901 ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
      961 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     1021 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccaccgg tcgtctccaa
     1081 cgaccacctc gtcgccttgg cctgcctcgg cggacgtcct gccatggatg cagtgaaaaa
     1141 gggattgccg cacgcgccgg aattgatcag aagagtcaat cgccgtattg gcgaacgcac
     1201 gtcccatcgc gttgccggat cccaactagt caaaagtgaa ctggaggaga agaaatctga
     1261 acttcgtcat aaattgaaat atgtgcctca tgaatatatt gaattaattg aaattgccag
     1321 aaattccact caggatagaa ttcttgaaat gaaggtaatg gaatttttta tgaaagttta
     1381 tggatataga ggtaaacatt tgggtggatc aaggaaaccg gacggagcaa tttatactgt
     1441 cggatctcct attgattacg gtgtgatcgt ggatactaaa gcttatagcg gaggttataa
     1501 tctgccaatt ggccaagcac gtgaaatgca acgatatgtc gaagaaaatc aaacacgaaa
     1561 caaacatatc aaccctaatg aatggtggaa agtctatcca tcttctgtaa cggaatttaa
     1621 gtttttattt gtgagtggtc actttaaagg aaactacaaa gctcagctta cacgattaaa
     1681 tcatatcact aattgtaatg gagctgttct tagtgtagaa gagcttttaa ttggtggaga
     1741 aatgattaaa gccggcacat taaccttaga ggaagtgcgc cggaaattta ataacggcga
     1801 gataaacttt taatctagaa ctatagtgag tcgtattacg tagatccaga catgataaga
     1861 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1921 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1981 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa
     2041 ttcgcggccg cggcgccaat gcattgggcc cggtacgtac ccagcttttg ttccctttag
     2101 tgagggttaa ttgcgcgctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     2161 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     2221 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     2281 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
     2341 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     2401 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     2461 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     2521 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     2581 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     2641 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     2701 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     2761 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     2821 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     2881 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     2941 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     3001 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     3061 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     3121 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     3181 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     3241 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     3301 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     3361 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     3421 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     3481 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     3541 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     3601 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     3661 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     3721 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     3781 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     3841 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     3901 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     3961 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     4021 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     4081 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     4141 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     4201 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     4261 acatttcccc gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt
     4321 taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     4381 ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     4441 cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     4501 gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac
     4561 taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     4621 tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     4681 cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     4741 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     4801 tattacgcca gtcgaccata gccaattcaa tatggcgtat atggactcat gccaattcaa
     4861 tatggtggat ctggacctgt gccaattcaa tatggcgtat atggactcgt gccaattcaa
     4921 tatggtggat ctggacccca gccaattcaa tatggcggac ttggcaccat gccaattcaa
     4981 tatggcggac ttggcactgt gccaactggg gaggggtcta cttggcacgg tgccaagttt
     5041 gaggaggggt cttggccctg tgccaagtcc gccatattga attggcatgg tgccaataat
     5101 ggcggccata ttggctatat gccaggatca atatataggc aatatccaat atggccctat
     5161 gccaatatgg ctattggcca ggttcaatac tatgtattgg ccctatgcca tatagtattc
     5221 catatatggg ttttcctatt gacgtagata gcccctccca atgggcggtc ccatatacca
     5281 tatatggggc ttcctaatac cgcccatagc cactccccca ttgacgtcaa tggtctctat
     5341 atatggtctt tcctattgac gtcatatggg cggtcctatt gacgtatatg gcgcctcccc
     5401 cattgacgtc aattacggta aatggcccgc ctggctcaat gcccattgac gtcaatagga
     5461 ccacccacca ttgacgtcaa tgggatggct cattgcccat tcatatccgt tctcacgccc
     5521 cctattgacg tcaatgacgg taaatggccc acttggcagt acatcaatat ctattaatag
     5581 taacttggca agtacattac tattggaagg acgccagggt acattggcag tactcccatt
     5641 gacgtcaatg gcggtaaatg gcccgcgatg gctgccaagt acatccccat tgacgtcaat
     5701 ggggaggggc aatgacgcaa atgggcgttc cattgacgta aatgggcggt aggcgtgcct
     5761 aatgggaggt ctatataagc aatgctcgtt tagggaac
//

载体图谱质粒图谱pdf版和相关资料下载

载体质粒应用举例

  • 上一篇: pENTR/pTER+ (430-1)
  • 下一篇: pCAG-T7-TALEN(Sangamo)-FokI-ELD-Destination
分享到:
推荐 7

全部载体分类

相关载体

  • pFLAG-CMV-4
  • pAMβ1
  • pCDM8
  • pMK-1
  • pG525
  • pHT43
  • pET-41a(+)
  • pCMV-FLAG-N
  • pET-24b(+)
  • pIJ702-del35-55
  • pSilencer 4.1-CMV neo
  • pEE12.4
  • pCYM3
  • pBP103
  • pIZ/V5-His
  • pENTR4-FLAG
  • pSK (BEC/PP:3,3)
  • pAAV-CAG-RFP
  • pSUPERIOR.puro
  • pTet-Off
  • pLB3A
  • pHM12
  • pLVX-IRES-tdTomato-Neo
  • p3
  • pUB110
  • pGH101
  • pET-47b(+)
  • pFastBac1-His-C
  • PX392
  • pcDNA6.2/nTC-Tag-DEST
  • pCMV-Tag 4A
  • ppArt.chl-bgl1-483
  • pSVc-myc-1
  • YCp19
  • pBAD30
  • pBI221-CFP
  • pSC101
  • pCDH-MCS-T2A-Puro-MSCV
  • pBLOCK-iT6-DEST
  • pBT-5
  • pCR13-3
  • pCgpv
  • pLenti CMV/TO Puro DEST (670-1)
  • pEBFP-N1
  • pfdB2
  • pRSET A/M
  • pOM8
  • pET-25b(+)
  • pLVX-DD-AmCyan1 Reporter
  • pAAV-hrGFP
  • pLVX-DsRed-Express2-C1
  • pTWIN2
  • pHer.aur-bgl1-474
  • pTrxFus
  • pEarleyGate202
  • pTransketo-TA
  • pcDNA6/V5-His/LacZ
  • pET-41c(+)
  • pHR40
  • pEZZ18
  • pDP8rs
  • pSilencer 3.1-H1 neo
  • pAcGFP1-N In-Fusion Ready
  • pdKeima-Red-S1
  • pCold-SUMO
  • pMA5MCS
  • pTrc99a
  • p10A1
  • pMH3/3097
  • pC22
  • pBlueBacHis2 C
  • pcDNA6.2-GW/EmGFP-miR
  • pECFP-Nuc
  • pDEST14
  • pSUP5011::Tn5-mob
  • p304
  • pSico PGK Puro
  • pLF3
  • pFV17
  • pRme-MVII/1a to pRme-MVII/1g
  • pCMS-EGFP
  • pWW20
  • pDP1rs
  • pPGV2
  • pcambia35s-ECFP
  • pXMJ19
  • pTP-4
  • pJLA502
  • pAAV-tre-mcherry
  • pGEX-Bgl3082(2)
  • pMUTIN-HA
  • pG2-vr
  • pHBLV-CMV-MCS-IRES-Puro
  • pLVX-IRES-Neo
  • pEGFP-N1
  • pTRKH3-ldhGFP
  • SARS Frag. 14 pCRII-TOPO
  • pAc5.1B-EGFP
  • pHY300
  • pCPOT
  • BPK2139
  • pMV306hsp+Lux
  • pRL1063a
  • pUB_smFLAG_KDM5B_MS2
  • pCasKP-apr
  • pDONR223_PIK3CA_p.H1047R
  • 3xHA-miniTurbo-NLS_pCDNA3
  • pcDNA3.1 EYFP H148Q/I152L
  • pmU6-gRNA
  • PL-SIN-PGK-EGFP
  • pEVOL-AckRS
  • pTRE-Tight-MitoTimer
  • AID-BE3
  • pLVX-puro_G-CEPIA1er
  • pGEX6p-1-BCCP-PupE
  • pLenti-GFP1-9
  • pETcon(-)
  • pQCasTns(Ptr)-IS186
  • pET28a-SnoopCatcher
  • NES-EGFP-cPHx1
  • pGreen2x35SLUC
  • NGFR
  • pAtetO_6xHis-Klentaq
  • pDIRECT_23C
  • pBHt2
  • pVDL9.3
  • pLenti CMV/TO GFP-Zeo DEST (719-1)
  • pActPL-Gal4DBD
  • HA-HA-AR-NTD
  • pBABe-HA-ER-IPpoI
  • secreted BirA-Flag
  • pCT5-bac2.0
  • P307-FBP_6
  • pBK-YEE-BE3
  • pC0048-CMV-dPspCas13b-longlinker-ADAR2DD
  • pMSCV-LTR-dCas9-VP64-BFP
  • pLL39
  • BL21(DE3)-R3-pRARE2
  • pCDNA3-Flag MKK6
  • pGL2Basic-EcadK1
  • pXY1
  • G1333 DddAtox-C–dSpCas9
  • piRFP
  • pHR_Gal4UAS_Pembrolizumab_heavychain_T2A
  • pMXs-hKLF4
  • T7-TPase
  • pREDCas9
  • Nme2Cas9_pCDest
  • pUC-UAS1B8-TEF(136)
  • pFA6a-hphMX-tetO-Pcyc1-3xFLAG
  • pMaster1
  • pBFC0619
  • pcDNA3.1-CMV-CFP;UBC-Cre25nt
  • pMT525
  • PA-mCherry1-C1
  • pmiRFP720-N1
  • pCgVchcrRNA
  • pKOdeltagalK (Hyg)
  • pMSCV-IRES-GFP II
  • pLP-LNCX-PIK3CA-WT
  • v3em-Nterm-PE2max
  • FH-TET3-pEF
  • Dendra2-H1-10
  • DHFR
  • CMV-SEAP
  • pCE27
  • pCS2+HyPer7
  • pcDNA5/FRT/TO-GAlphaGustducin-RLuc8
  • pEGFP hsp70
  • pRW006-SM-destination-pro35S-Cas9
  • hRad51(S208E-A209D)–Cas9(D10A)
  • pcDNA GNSTM-3-Flag-10-Lamp2b-HA
  • pST1374-NLS-flag-linker-Cas9
  • pSBbi-Pur
  • mApple-N1
  • 3XFlag-pA-Tn5-Fl
  • AAVS1-Blasticidin-CAG-Flpe-ERT2
  • pHAGE2-TetOminiCMV-SKM
  • pCMV-PEmax
  • PABE-1
  • pSLAX13 (CT#232)
  • pAAV-Ef1a-DIO EYFP
  • CMV-mito-R-GECO1
  • C1(1-29)-TurboID-V5_pCDNA3
  • pORANGE Cloning template vector
  • pGL4.0-TERT WT
  • pN1-GCaMP6m-XC
  • pSR58.6
  • pAAV-EF1a-DIO-mCherry
  • pTE4497
  • pG3H-U3Ub
  • pET30a-His6-LOXCAT
  • sfGFP-Cx43K258stop
  • pET42b-BE3
  • pCMV6 BDNF
  • pcDNA3 HA Arf6 DN T27N
  • pUCDM
  • pHSE401
  • p6659 MSCV-IP N FlagHA HPV16E6
  • pNI10

相关文章

相关问答

关于我们 加入生物风 网站地图 Sitemap
Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng 沪ICP备2021037978号-1
欢迎您的访问和咨询....

深圳SEO优化公司让您放心省心企业网站优化南通网站优化服务商罗湖付费网站优化怎么做眉山网站排名优化实力强的网站关键词优化咨询电话网站推广优化方案招聘网站用户体验度页面优化机械网站优化优势寒亭网站关键词优化佛山网站优化网站优化内链优化延安网站优化如何济南卫浴行业网站优化推广技巧吉林广电网站优化检修网站推广优化挥性冫云速捷网站的优化对公司的作用网站的优化舒心火4星完美韶关建材网站seo优化网站搜索优化心细火23星网站优化要从哪些方面做南京营销网站设计优化价格渤海新区网站关键词优化河南网站优化有哪些网站长期优化没排名网站优化问题产生的原因保定优化网站丽水网站建设及优化铁岭网站长尾关键词优化嘉定区公司官方网站优化网站首页优化方案歼20紧急升空逼退外机英媒称团队夜以继日筹划王妃复出草木蔓发 春山在望成都发生巨响 当地回应60岁老人炒菠菜未焯水致肾病恶化男子涉嫌走私被判11年却一天牢没坐劳斯莱斯右转逼停直行车网传落水者说“没让你救”系谣言广东通报13岁男孩性侵女童不予立案贵州小伙回应在美国卖三蹦子火了淀粉肠小王子日销售额涨超10倍有个姐真把千机伞做出来了近3万元金手镯仅含足金十克呼北高速交通事故已致14人死亡杨洋拄拐现身医院国产伟哥去年销售近13亿男子给前妻转账 现任妻子起诉要回新基金只募集到26元还是员工自购男孩疑遭霸凌 家长讨说法被踢出群充个话费竟沦为间接洗钱工具新的一天从800个哈欠开始单亲妈妈陷入热恋 14岁儿子报警#春分立蛋大挑战#中国投资客涌入日本东京买房两大学生合买彩票中奖一人不认账新加坡主帅:唯一目标击败中国队月嫂回应掌掴婴儿是在赶虫子19岁小伙救下5人后溺亡 多方发声清明节放假3天调休1天张家界的山上“长”满了韩国人?开封王婆为何火了主播靠辱骂母亲走红被批捕封号代拍被何赛飞拿着魔杖追着打阿根廷将发行1万与2万面值的纸币库克现身上海为江西彩礼“减负”的“试婚人”因自嘲式简历走红的教授更新简介殡仪馆花卉高于市场价3倍还重复用网友称在豆瓣酱里吃出老鼠头315晚会后胖东来又人满为患了网友建议重庆地铁不准乘客携带菜筐特朗普谈“凯特王妃P图照”罗斯否认插足凯特王妃婚姻青海通报栏杆断裂小学生跌落住进ICU恒大被罚41.75亿到底怎么缴湖南一县政协主席疑涉刑案被控制茶百道就改标签日期致歉王树国3次鞠躬告别西交大师生张立群任西安交通大学校长杨倩无缘巴黎奥运

深圳SEO优化公司 XML地图 TXT地图 虚拟主机 SEO 网站制作 网站优化