TCP10L Polyclonal antibody

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IHC, WB,ELISA

Conjugate

Unconjugated

Cat No : 13709-1-AP

Print datasheet

Synonyms

C21orf77, PRED77, t complex 10 (mouse) like, T complex protein 10A 2, TCP10 like, TCP10A 2, TCP10L

概述

Print datasheet

MSDS

WB analysis of mouse testis using 13709-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 13709-1-AP (TCP10L antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 13709-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 13709-1-AP (TCP10L antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 13709-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 13709-1-AP (TCP10L antibody) at dilution of 1:50 (under 40x lens).
IF Staining of HeLa using 13709-1-AP
Immunofluorescent analysis of Hela cells, using 13709-1-AP and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Size

Positive WB detected in	mouse testis tissue
Positive IHC detected in	human testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	Hela cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
Immunofluorescence (IF)	IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

13709-1-AP targets TCP10L in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications	IF/ICC, IHC, WB,ELISA
Tested Reactivity	human, mouse
Immunogen	TCP10L fusion protein Ag4646 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	t-complex 10 (mouse)-like
Synonyms	C21orf77, PRED77, t complex 10 (mouse) like, T complex protein 10A 2, TCP10 like, TCP10A 2, TCP10L
Calculated Molecular Weight	215 aa, 24 kDa
Observed Molecular Weight	24 kDa
GenBank Accession Number	BC033855
Gene Symbol	TCP10L
Gene ID (NCBI)	140290
RRID	AB_2255905
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TDR4
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Product Specific Protocols
WB protocol for TCP10L antibody 13709-1-AP	Download protocol
IHC protocol for TCP10L antibody 13709-1-AP	Download protocol
IF protocol for TCP10L antibody 13709-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols