- Featured Product
- KD/KO Validated
Cyclin A2 Monoclonal antibody
Cyclin A2 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human and More (2)
Applications
IF/ICC, IHC, WB, ELISA
Conjugate
Unconjugated
Publications
23
CloneNo.
1C6C11
Cat No : 66391-1-Ig
Print datasheet
Synonyms
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, HeLa cells, HEK-293 cells, Jurkat cells, Raji cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells, MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy CCNA2 Ready-To-Use IHC Kit. CCNA2 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 20 publications below |
| IF | See 3 publications below |
产品信息
66391-1-Ig targets Cyclin A2 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | IF/ICC, IHC, WB, ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Immunogen | Cyclin A2 fusion protein Ag12765 种属同源性预测 |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | cyclin A2 |
| Synonyms | CCN1, CCNA, CCNA2, Cyclin A, Cyclin A2, cyclinA2 |
| Calculated Molecular Weight | 432 aa, 49 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC104783 |
| Gene Symbol | CCNA2 |
| Gene ID (NCBI) | 890 |
| RRID | AB_2881767 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P20248 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CCNA2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. CCNA2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyclin A2 antibody 66391-1-Ig | Download protocol |
| IHC protocol for Cyclin A2 antibody 66391-1-Ig | Download protocol |
| IF protocol for Cyclin A2 antibody 66391-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
- All (23)
- WB (20)
- IF (3)
| Species | Application | Title |
|---|---|---|
Dev Cell Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation. View Article | ||
Oncogene KCTD12 promotes tumorigenesis by facilitating CDC25B/CDK1/Aurora A-dependent G2/M transition. View Article | ||
Elife METTL3 promotes homologous recombination repair and modulates chemotherapeutic response in breast cancer by regulating the EGF/RAD51 axis. View Article | ||
J Cell Mol Med Cell division cycle associated 8: A novel diagnostic and prognostic biomarker for hepatocellular carcinoma View Article | ||
Cell Cycle Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D. View Article | ||
Molecules A Potential Anti-Glioblastoma Compound LH20 Induces Apoptosis and Arrest of Human Glioblastoma Cells via CDK4/6 Inhibition View Article |
